Recent Progress in Light Sheet Microscopy for Biological Applications
| Autor: | Peilin Chen, Frances Camille M. Wu, Bi-Chang Chen, Feby Wijaya Pratiwi, Krishnendu Chatterjee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Super-resolution microscopy Cellular imaging Cytological Techniques Optical Imaging Nanotechnology Acquisition rate law.invention Developmental dynamics Mice 03 medical and health sciences 030104 developmental biology Optical imaging Microscopy Fluorescence Confocal microscopy law Light sheet fluorescence microscopy Animals Humans Spatiotemporal resolution Instrumentation Cells Cultured Spectroscopy |
| Zdroj: | Applied Spectroscopy. 72:1137-1169 |
| ISSN: | 1943-3530 0003-7028 |
| DOI: | 10.1177/0003702818778851 |
| Popis: | The introduction of light sheet fluorescence microscopy (LSFM) has overcome the challenges in conventional optical microscopy. Among the recent breakthroughs in fluorescence microscopy, LSFM had been proven to provide a high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and minuscule levels of phototoxic and photodamage effects. The aforementioned auspicious properties are crucial in the biomedical and clinical research fields, covering a broad range of applications: from the super-resolution imaging of intracellular dynamics in a single cell to the high spatiotemporal resolution imaging of developmental dynamics in an entirely large organism. In this review, we provided a systematic outline of the historical development of LSFM, detailed discussion on the variants and improvements of LSFM, and delineation on the most recent technological advancements of LSFM and its potential applications in single molecule/particle detection, single-molecule super-resolution imaging, imaging intracellular dynamics of a single cell, multicellular imaging: cell–cell and cell–matrix interactions, plant developmental biology, and brain imaging and developmental biology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|