Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
| Autor: | Giovana Souza Branco, Renata Guimarães Moreira, Carlos Eduardo Tolussi, Fabiana Laura Lo Nostro, Gabriela Brambila-Souza, Filipe Guilherme Andrade de Godoi, Marcela Muñoz-Peñuela, Aline Dal’Olio Gomes |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Anhembi-Morumbi, Universidade Estadual Paulista (Unesp), CONICET-UBA |
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Diclofenac Physiology Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology Endocrine Disruptors 010501 environmental sciences Characiformes Toxicology 01 natural sciences Biochemistry Muscle hypertrophy chemistry.chemical_compound Vitellogenin Caffeine Internal medicine Testis medicine Animals Endocrine system Testosterone FÁRMACOS Endocrine disrupting chemicals 0105 earth and related environmental sciences 021110 strategic defence & security studies Estradiol biology Characidae Cell Biology General Medicine biology.organism_classification Sperm Fish Endocrinology Liver Steroidogenesis chemistry Vitellogenin a biology.protein Pharmaceuticals Water Pollutants Chemical medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-12T01:55:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 Companhia Energética de São Paulo Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology. Departamento de Fisiologia Instituto de Biociências Universidade de São Paulo, Rua do Matão, Trav.14, n° 321 Universidade Anhembi-Morumbi, Campus Mooca UNESP - Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP) Lab. de Ecotoxicología Acuática Departamento de Biodiversidad y Biología Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires & IBBEA CONICET-UBA UNESP - Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP) CAPES: 001 CNPq: 130190/2017-9 FAPESP: 2014/16320-7 FAPESP: 2017/07139-5 FAPESP: 2017/11530-1 |
| Databáze: | OpenAIRE |
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