Gene expression and growth factor analysis in early nerve regeneration following segmental nerve defect reconstruction with a mesenchymal stromal cell-enhanced decellularized nerve allograft
| Autor: | Allen T. Bishop, Nadia Rbia, Liselotte F. Bulstra, Patricia F. Friedrich, Alexander Y. Shin, Tim H.J. Nijhuis |
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| Přispěvatelé: | Plastic and Reconstructive Surgery and Hand Surgery |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Wallerian degeneration
Decellularization biology Nerve allograft business.industry Regeneration (biology) Mesenchymal stem cell lcsh:Surgery lcsh:RD1-811 030230 surgery medicine.disease Nerve conduction velocity Cell biology Experimental 03 medical and health sciences surgical procedures operative 0302 clinical medicine Neurotrophic factors 030220 oncology & carcinogenesis ComputingMethodologies_DOCUMENTANDTEXTPROCESSING medicine biology.protein Surgery business Neurotrophin |
| Zdroj: | Plastic and Reconstructive Surgery-Global Open, 8(1):e2579. Lippincott Williams & Wilkins Plastic and Reconstructive Surgery, Global Open, Vol 8, Iss 1, p e2579 (2020) Plastic and Reconstructive Surgery Global Open |
| ISSN: | 2169-7574 |
| Popis: | Supplemental Digital Content is available in the text. Background: The purpose of this study was to evaluate the molecular mechanisms underlying nerve repair by a decellularized nerve allograft seeded with adipose-derived mesenchymal stromal cells (MSCs) and compare it to the unseeded allograft and autograft nerve. Methods: Undifferentiated MSCs were seeded onto decellularized nerve allografts and used to reconstruct a 10 mm gap in a rat sciatic nerve model. Gene expression profiles of genes essential for nerve regeneration and immunohistochemical staining (IHC) for PGP9.5, NGF, RECA-1, and S100 were obtained 2 weeks postoperatively. Results: Semi-quantitative RT-PCR analysis showed that the angiogenic molecule VEGFA was significantly increased in seeded allografts, and transcription factor SOX2 was downregulated in seeded allografts. Seeded grafts showed a significant increase in immunohistochemical markers NGF and RECA-1, when compared with unseeded allografts. Conclusions: MSCs contributed to the secretion of trophic factors. A beneficial effect of the MSCs on angiogenesis was found when compared with the unseeded nerve allograft, but implanted MSCs did not show evidence of differentiation into Schwann cell-like cells. |
| Databáze: | OpenAIRE |
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