Activated Syndecan-1 shedding contributes to mice colitis induced by dextran sulfate sodium
| Autor: | Xiaoyun Xie, Ye Chen, Yu-Gang Song, Xia Wang, Xianfei Wang, Shaoheng Zhang |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Physiology Ratón medicine.drug_class Colon animal diseases Down-Regulation Pharmacology digestive system Syndecan 1 chemistry.chemical_compound Mice medicine Animals Colitis Intestinal Mucosa Mice Inbred BALB C business.industry Heparin Anticoagulant Dextran Sulfate Gastroenterology Heparan sulfate medicine.disease Ulcerative colitis digestive system diseases Interleukin-10 Up-Regulation carbohydrates (lipids) stomatognathic diseases Ectodomain chemistry Immunology Syndecan-1 business medicine.drug Interleukin-1 |
| Zdroj: | Digestive diseases and sciences. 56(4) |
| ISSN: | 1573-2568 |
| Popis: | Syndecan-1(Sdc1) plays important roles in many steps of inflammatory responses. In ulcerative colitis patients, decreased Sdc1 expression was observed and Sdc1 analogue heparin could improve the disease course. A better understanding of how Sdc1 functions in colitis will benefit the disease intervention. To evaluate the role of Sdc1 in dextran sulfate sodium (DSS)-induced colitis. BALB/c mice were grouped randomly into control, DSS, and heparin+DSS. The DSS group was given 4% DSS orally and heparin+DSS group was given 4% DSS with heparin (enoxaparin) subcutaneously, while the control was given distilled water orally. All mice were killed at day 7. Disease activities, histopathological changes, membrane-bound and free Sdc1 level and mRNA expression of Sdc1, IL-1, and IL-10 in colon mucosa were detected. Significant colitis was observed in the DSS group, but disease activity index and histological score showed significant lower in the heparin+DSS group than those in the DSS group. Compared to the control group, decreased Sdc1 protein expression was detected in colon mucosa of DSS-induced colitis while Sdc1 ectodomain level in serum was much higher. Inhibited Sdc1 ectodomain shedding was detected in the heparin+DSS group compared to the DSS group. RT-PCR demonstrated that both IL-1 and IL-10 expression were up-regulated in DSS-induced colitis while heparin lessened the up-regulation extent. Sdc1 shedding is activated in DSS-induced colitis and heparin, which mimics Sdc1 functions, relieves colitis severity by inhibiting Sdc1 shedding and down-regulating cytokines expression. |
| Databáze: | OpenAIRE |
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