Kinetics of Prion Protein Accumulation in the CNS of Mice with Experimental Scrapie
| Autor: | Darlene Groth, Marilyn Torchia, Jörg Tatzelt, Stanley B. Prusiner, Stephen J. DeArmond |
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| Rok vydání: | 1999 |
| Předmět: |
Time Factors
PrPSc Proteins Prions Ratón animal diseases Blotting Western Detergents Kinetics Scrapie Spleen Biology Pathology and Forensic Medicine Microbiology Mice Cellular and Molecular Neuroscience medicine Animals Tissue Distribution Prion protein Western immunoblotting Inoculation Proteolytic enzymes Brain General Medicine Virology PrP 27-30 Protein nervous system diseases medicine.anatomical_structure Solubility Neurology Neurology (clinical) |
| Zdroj: | Journal of Neuropathology & Experimental Neurology. 58:1244-1249 |
| ISSN: | 1554-6578 0022-3069 |
| Popis: | The kinetics of PrP(Sc) and insoluble PrP accumulation in the spleens and brains of CD-1 mice were studied. The mice were inoculated intracerebrally with RML prions and euthanized at various times between inoculation and the onset of illness at approximately 130 days. Protease-resistant PrP(Sc), PrP 27-30, was first detected in brain by histoblotting 49 days after inoculation and by Western immunoblotting at 70 days. In spleen, PrP 27-30 was first detected by Western immunoblotting at 28 days after inoculation. Like PrP 27-30, substantial increases in detergent-insoluble PrP were first detected at 70 days after inoculation in brain and 28 days in spleen. In addition, a progressive increase in detergent-soluble PrP was detected beginning 70 days after inoculation. Further characterization of detergent soluble and insoluble PrP with respect to protease-sensitive PrP(Sc) and prion infectivity will be of considerable interest. |
| Databáze: | OpenAIRE |
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