Localization and Characterization of 170 BAC-Derived Clones and Mapping of 94 Microsatellites in the Hessian Fly
| Autor: | Yan M. Crane, Brandon J. Schemerhorn, Rajat Aggarwal, Philip K. Morton, Thiago R Benatti |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Chromosomes
Artificial Bacterial Molecular Sequence Data Gene mapping Genetics medicine Animals Genomic library Molecular Biology Mayetiola destructor Genetics (clinical) Gene Library Bacterial artificial chromosome Polytene chromosome Base Sequence biology medicine.diagnostic_test Diptera Physical Chromosome Mapping Computational Biology Genetic Variation food and beverages Sequence Analysis DNA biology.organism_classification Genetics Population Microsatellite Microsatellite Repeats Biotechnology Fluorescence in situ hybridization |
| Zdroj: | Journal of Heredity. 100:790-797 |
| ISSN: | 1465-7333 0022-1503 |
| DOI: | 10.1093/jhered/esp045 |
| Popis: | Ninety-four microsatellites from enriched genomic libraries of Hessian fly (Hf, Mayetiola destructor [Say]) were localized to 170 cognate clones in an Hf bacterial artificial chromosome (BAC) library. These microsatellite-positive BAC clones were physically mapped to polytene chromosomes by fluorescent in situ hybridization. The mapped microsatellite loci can be used to study the genetic diversity and population structure of Hf. |
| Databáze: | OpenAIRE |
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