The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells
| Autor: | Wenyi Lv, Hao Huang, Meiling Huang, Hongfeng Tang, Ye Gao, Hanyue Xiao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Aging
Article Subject Trichophyton rubrum Arginine Nitric Oxide Biochemistry Cell Line Nitric oxide chemistry.chemical_compound Catecholamines Microscopy Electron Transmission Trichophyton Humans Enzyme Inhibitors Imidazolines skin and connective tissue diseases chemistry.chemical_classification Reactive oxygen species omega-N-Methylarginine NADPH oxidase QH573-671 integumentary system biology NADPH Oxidases Cell Biology General Medicine biology.organism_classification Molecular biology Nitric oxide synthase HaCaT chemistry Cell culture Microscopy Electron Scanning biology.protein Nitric Oxide Synthase Reactive Oxygen Species Cytology Asymmetric dimethylarginine Research Article Peptide Hydrolases |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2020/8548619 |
| Popis: | Trichophyton rubrum (T. rubrum) is one of the most important agents of dermatophyte infection in humans. The aim of this experiment was to evaluate the effect of HaCaT cells on T. rubrum, investigate the responsible mechanism of action, and explore the role of reactive oxygen species (ROS) and nitric oxide (NO) in the inhibition of T. rubrum growth by HaCaT cells. The viability of fungi treated with HaCaT cells alone and with HaCaT cells combined with pretreatment with the NADPH oxidase inhibitor (DPI) or the nitric oxide synthase (NOS) inhibitor L-NMMA was determined by enumerating the colony-forming units. NOS, ROS, and NO levels were quantified using fluorescent probes. The levels of the NOS inhibitor asymmetric dimethylarginine (ADMA) were determined by enzyme-linked immunosorbent assay (ELISA). Micromorphology was observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In addition, fungal keratinase activity was assessed by measuring dye release from keratin azure. In vitro fungal viability, keratinase activity, and ADMA content decreased after HaCaT cell intervention, whereas the levels of ROS, NO, and NOS increased. The micromorphology was abnormal. Fungi pretreated with DPI and L-NMMA exhibited opposite effects. HaCaT cells inhibited the growth and pathogenicity of T. rubrum in vitro. A suggested mechanism is that ROS and NO play an important role in the inhibition of T. rubrum growth by HaCaT cells. |
| Databáze: | OpenAIRE |
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