Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
| Autor: | Ricardo Harakava, E. B. Rivas, Estevão Cardoso de Almeida Bôdi, Fábio Gregori, Marcos Cesar Gonçalves |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Agriculture (General) Tulip breaking virus phylogeny Virus S1-972 law.invention Cucumber mosaic virus 03 medical and health sciences Lily symptomless virus Phylogenetics law Plant virus RT-PCR parasitic diseases Quarantine biology Lilium Phylogenetic tree recombination analysis Lily mottle virus biology.organism_classification Virology 030104 developmental biology VÍRUS DE PLANTAS Animal Science and Zoology Agronomy and Crop Science |
| Zdroj: | Pesquisa Agropecuária Brasileira, Vol 51, Iss 5, Pp 615-622 (2016) Pesquisa Agropecuária Brasileira v.51 n.5 2016 Pesquisa Agropecuária Brasileira Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0100-204X |
| DOI: | 10.1590/s0100-204x2016000500023 |
| Popis: | The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus - LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT-PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV-infected lilies and nine fragments of 600 nt from LSV-infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV). |
| Databáze: | OpenAIRE |
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