The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides
| Autor: | La aied Prapanthadara, Nongkran Lumjuan, Shavanthi Rajatileka, Gareth Lycett, Pradya Somboon, Jureeporn Wicheer, Posri Leelapat, Hilary Ranson, Donch Changsom |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Population Molecular Sequence Data Lyases Aedes aegypti Biochemistry DDT Insecticide Resistance chemistry.chemical_compound Aedes parasitic diseases Nitriles Pyrethrins Animals Amino Acid Sequence Binding site education Molecular Biology Peptide sequence Gene Glutathione Transferase education.field_of_study Pyrethroid biology biology.organism_classification Deltamethrin chemistry Gene Expression Regulation Insect Science Female |
| Zdroj: | Insect biochemistry and molecular biology. 41(3) |
| ISSN: | 1879-0240 |
| Popis: | The Epsilon glutathione transferase (GST) class in the dengue vector Aedes aegypti consists of eight sequentially arranged genes spanning 53,645 bp on super contig 1.291, which maps to chromosome 2. One Epsilon GST, GSTE2, has previously been implicated in conferring resistance to DDT. The amino acid sequence of GSTE2 in an insecticide susceptible and a DDT resistant strain differs at five residues two of which occur in the putative DDT binding site. Characterization of the respective recombinant enzymes revealed that both variants have comparable DDT dehydrochlorinase activity although the isoform from the resistant strain has higher affinity for the insecticide. GSTe2 and two additional Epsilon GST genes, GSTe5 and GSTe7, are expressed at elevated levels in the resistant population and the recombinant homodimer GSTE5-5 also exhibits low levels of DDT dehydrochlorinase activity. Partial silencing of either GSTe7 or GSTe2 by RNA interference resulted in an increased susceptibility to the pyrethroid, deltamethrin suggesting that these GST enzymes may also play a role in resistance to pyrethroid insecticides. |
| Databáze: | OpenAIRE |
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