Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in Saccharomyces cerevisiae
| Autor: | Amy E. Ikui, Keiko Kono, Lea Schroeder, Amr Al-Zain, Makoto Nakanishi |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Replication Cell cycle checkpoint Cell division DNA damage Saccharomyces cerevisiae Cell membrane 03 medical and health sciences 0302 clinical medicine Cyclin-dependent kinase Cell Wall Cyclins medicine CHEK1 DNA Fungal Multidisciplinary biology Cell Membrane DNA replication G1 Phase Cell Cycle Checkpoints G2-M DNA damage checkpoint Biological Sciences Cyclin-Dependent Kinases Cell biology 030104 developmental biology medicine.anatomical_structure biology.protein 030217 neurology & neurosurgery |
| Popis: | Cellular wound healing or the repair of plasma membrane/cell wall damage (plasma membrane damage) occurs frequently in nature. Although various cellular perturbations, such as DNA damage, spindle misalignment, and impaired daughter cell formation, are monitored by cell cycle checkpoint mechanisms in budding yeast, whether plasma membrane damage is monitored by any of these checkpoints remains to be addressed. Here, we define the mechanism by which cells sense membrane damage and inhibit DNA replication. We found that the inhibition of DNA replication upon plasma membrane damage requires GSK3/Mck1-dependent degradation of Cdc6, a component of the prereplicative complex. Furthermore, the CDK inhibitor Sic1 is stabilized in response to plasma membrane damage, leading to cell integrity maintenance in parallel with the Mck1-Cdc6 pathway. Cells defective in both Cdc6 degradation and Sic1 stabilization failed to grow in the presence of plasma membrane damage. Taking these data together, we propose that plasma membrane damage triggers G1 arrest via Cdc6 degradation and Sic1 stabilization to promote the cellular wound healing process. |
| Databáze: | OpenAIRE |
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