Diversity of Ampeloviruses in Mealybug and Soft Scale Vectors and in Grapevine Hosts from Leafroll-Affected Vineyards
| Autor: | Gregory M. Loeb, H C Hoch, Patricia Marsella-Herrick, Timothy E. Martinson, Marc Fuchs |
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| Rok vydání: | 2009 |
| Předmět: |
Insecta
DNA Plant New York Zoology Plant Science Pulvinaria innumerabilis Virus Plant Viruses law.invention law Phylogenetics Plant virus Botany Animals Vitis Mealybug Closteroviridae Phylogeny Polymerase chain reaction Plant Diseases biology Reverse Transcriptase Polymerase Chain Reaction Biodiversity biology.organism_classification Insect Vectors Pseudococcus maritimus Agronomy and Crop Science |
| Zdroj: | Phytopathology®. 99:1177-1184 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-99-10-1177 |
| Popis: | The occurrence and diversity of Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) in the soft scales Parthenolecanium corni and Pulvinaria innumerabilis and in the mealybug Pseudococcus maritimus was determined in leafroll-affected vineyards in the Finger Lakes region of New York. Groups of 1 to 4 specimens were collected under loose grapevine bark and tested by reverse-transcription polymerase chain reaction (RT-PCR) for segments of the second diverged copy of the GLRaV-1 coat protein gene or GLRaV-3 heat-shock protein 70-homologue gene. Virus-specific RT-PCR products were amplified from immature insect vectors and adult mealybugs. Single viral amplicons were obtained mostly from immature vectors (35%, 30 of 85) and dual viral amplicons from immature (16%, 10 of 61) and adult (100%, 14 of 14) mealybugs, including individuals. These observations suggested a simultaneous uptake of GLRaV-1 and GLRaV-3 by individual mealybugs. Furthermore, a comparative nucleotide sequence analysis of viral amplicons from soft scales, mealybugs, and grapevines from which vectors were collected showed identical or highly similar haplotypes, indicating that uptake of GLRaV-1 and GLRaV-3 likely occurred by direct feeding of vectors on their host plants. |
| Databáze: | OpenAIRE |
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