Release of heparan sulfate from endothelial cells. Implications for pathogenesis of hyperacute rejection
| Autor: | Theodore R. Oegema, Agustin P. Dalmasso, Bonnie J. Lindman, Fritz H. Bach, Gregory M. Vercellotti, Jeffrey L. Platt |
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| Rok vydání: | 1990 |
| Předmět: |
Graft Rejection
Endothelium Swine Immunology Sulfur Radioisotopes Models Biological chemistry.chemical_compound Classical complement pathway medicine Immunology and Allergy Animals Humans Aorta Cells Cultured Glycosaminoglycans biology Sulfates Heparan sulfate Articles Blood Physiological Phenomena Blood proteins Cell biology Endothelial stem cell Kinetics medicine.anatomical_structure chemistry Proteoglycan Cell culture biology.protein Proteoglycans Endothelium Vascular Heparitin Sulfate Antibody |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Popis: | Heparan sulfate proteoglycan associated with endothelial cells in normal blood vessels inhibits intravascular coagulation and egress of blood cells and plasma proteins, key features of hyperacute rejection. It was shown herein that exposure of cultured porcine endothelium to human serum as a source of natural antibodies and complement caused cleavage and release of 5% of endothelial cell proteoglycans within 4 min and greater than 50% within 1 h. Proteoglycan release depended on activation of the classical complement pathway and preceded irreversible cell injury. These findings suggest that loss of endothelial cell proteoglycan may be a critical step in the pathogenesis of hyperacute rejection and in diseases involving humoral injury to endothelial cells. |
| Databáze: | OpenAIRE |
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