miR-20b regulates expression of proteinase-activated receptor-1 (PAR-1) thrombin receptor in melanoma cells
| Autor: | Lars Faxälv, Abdimajid Osman, Kjersti Claesson, Jan-Ingvar Jönsson, Amina Saleiban |
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| Rok vydání: | 2014 |
| Předmět: |
Dermatology
Biology General Biochemistry Genetics and Molecular Biology Metastasis Downregulation and upregulation Genes Reporter Cell Line Tumor Thrombin receptor microRNA medicine Humans Neoplasm Invasiveness Receptor PAR-1 Calcium Signaling Gene Silencing RNA Messenger RNA Neoplasm RNA Processing Post-Transcriptional Receptor 3' Untranslated Regions Melanoma neoplasms Regulation of gene expression Thrombin Transfection medicine.disease Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic MicroRNAs Oncology Cancer research |
| Zdroj: | Pigment Cell & Melanoma Research. 27:431-441 |
| ISSN: | 1755-1471 |
| Popis: | The proteinase-activated receptor 1 (PAR-1) plays a central role in melanoma progression and its expression level is believed to correlate with the degree of cancer invasiveness. Here, we show that PAR-1 is post-transcriptionally regulated by miR-20b microRNA in human melanoma cells. PAR-1 was found to be expressed in metastatic melanoma cells but was barely detectable in primary melanoma. By transducing primary melanoma cells with a lentivirus containing a 3'-UTR construct of PAR-1 mRNA, we could show that endogenous melanoma microRNAs interacted with PAR-1 3'-UTR and silenced a fused luciferase reporter. Transfection of an inhibitor against miR-20b into primary melanoma cells reversed this process. Finally, transfection of miR-20b mimic into metastatic melanoma cells caused downregulation of the luciferase reporter. We conclude that miR-20b regulates expression of melanoma PAR-1 receptor, which may explain the differential expression of PAR-1 observed in human melanoma. |
| Databáze: | OpenAIRE |
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