A Novel Pzg-NURF Complex Regulates Notch Target Gene Activity
| Autor: | Sabrina J. Kugler, Anja C. Nagel |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
NURF complex
Chromosomal Proteins Non-Histone Mutant Notch signaling pathway Cell Cycle Proteins medicine.disease_cause medicine Animals Drosophila Proteins Molecular Biology Genetics Regulation of gene expression Mutation Receptors Notch biology DNA Articles Cell Biology biology.organism_classification Signaling Clone Cells Chromatin Drosophila melanogaster Gene Expression Regulation Notch proteins Protein Binding |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e10-03-0212 |
| Popis: | The Putzig (Pzg) protein is associated with the NURF nucleosome remodeling complex, thereby promoting Notch target gene expression. Our findings suggest a novel Pzg-NURF complex that is responsible for the epigenetic regulation of Notch target genes. Drosophila putzig was identified as a member of the TRF2–DREF complex that is involved in core promoter selection. Additionally, putzig regulates Notch signaling, however independently of DREF. Here, we show that Putzig associates with the NURF complex. Loss of any NURF component including the NURF-specific subunit Nurf 301 impedes binding of Putzig to Notch target genes, suggesting that NURF recruits Putzig to these sites. Accordingly, Putzig can be copurified with any NURF member. Moreover, Nurf 301 mutants show reduced Notch target gene activity and enhance Notch mutant phenotypes. These data suggest a novel Putzig–NURF chromatin complex required for epigenetic activation of Notch targets. |
| Databáze: | OpenAIRE |
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