Vacuolar biogenesis and aquaporin expression at early germination of broad bean seeds
| Autor: | Colette Tournaire-Roux, G. V. Novikova, I. A. Sin’kevich, N. V. Obroucheva, S. V. Lityagina, Christophe Maurel |
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| Přispěvatelé: | Institute of Plant Physiology of Russian Academy of Sciences, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Program of the Presidium of Russian Academy of Sciences 'Cellular and Molecular Biology', Russian Federation for Basic Research [08-04-00416, 11-04-01139], CNRS/RAS cooperation agreement ['Aquaporins and seed germination'] [21263] |
| Rok vydání: | 2014 |
| Předmět: |
Physiology
Aquaporin Index Medicus Germination Plant Science Vacuole Biology Cell elongation Aquaporins Hypocotyl Genetics Radicle Storage protein [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Vicia faba minor Tonoplast aquaporins Water transport Plant Proteins chemistry.chemical_classification food and beverages Water Meristem Seed germination Vacuole biogenesis chemistry Biochemistry Seeds Vacuoles Gene expression |
| Zdroj: | Plant Physiology and Biochemistry Plant Physiology and Biochemistry, Elsevier, 2014, 82, pp.123-32. ⟨10.1016/j.plaphy.2014.05.014⟩ |
| ISSN: | 1873-2690 0981-9428 |
| DOI: | 10.1016/j.plaphy.2014.05.014⟩ |
| Popis: | International audience; A key event in seed germination is water uptake-mediated growth initiation in embryonic axes. Vicia faba var. minor (broad bean) seeds were used for studying cell growth, vacuolar biogenesis, expression and function of tonoplast water channel proteins (aquaporins) in embryonic axes during seed imbibition, radicle emergence and growth. Hypocotyl and radicle basal cells showed vacuole restoration from protein storage vacuoles, whereas de novo vacuole formation from provacuoles was observed in cells newly produced by root meristem. cDNA fragments of seven novel aquaporin isoforms including five Tonoplast Intrinsic Proteins (TIP) from three sub-types were amplified by PCR. The expression was probed using q-RT-PCR and when possible with isoform-specific antibodies. Decreased expression of TIP3s was associated to the transformation of protein storage vacuoles to vacuoles, whereas enhanced expression of a TIP2 homologue was closely linked to the fast cell elongation. Water channel functioning checked by inhibitory test with mercuric chloride showed closed water channels prior to growth initiation and active water transport into elongating cells. The data point to a crucial role of tonoplast aquaporins during germination, especially during growth of embryonic axes, due to accelerated water uptake and vacuole enlargement resulting in rapid cell elongation. |
| Databáze: | OpenAIRE |
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