Massively parallel sequencing analysis of nondegraded and degraded DNA mixtures using the ForenSeq™ system in combination with EuroForMix software
| Autor: | Wan-Chia Chung, Ming-Yih Wu, Hsiang-I Yin, Chih-Peng Lin, Tsang-Ming Ko, Tsui-Ting Lee, James Chun-I Lee, Hsiao-Lin Hwa |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Genotype Single-nucleotide polymorphism Biology 01 natural sciences Polymorphism Single Nucleotide Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Asian People Gene Frequency SNP Humans 030216 legal & forensic medicine Allele Genetics Likelihood Functions Massive parallel sequencing 010401 analytical chemistry DNA Degradation Necrotic High-Throughput Nucleotide Sequencing Sequence Analysis DNA DNA Fingerprinting humanities 0104 chemical sciences chemistry Microsatellite Female Amelogenin DNA Software Microsatellite Repeats |
| Zdroj: | International journal of legal medicine. 133(1) |
| ISSN: | 1437-1596 |
| Popis: | Massively parallel sequencing (MPS) technologies enable the simultaneous analysis of short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). MPS also enables the detection of alleles of the minor contributors in imbalanced DNA mixtures. In this study, 59 STRs (amelogenin, 27 autosomal STRs, 7 X-STRs, and 24 Y-STRs) and 94 identity-informative SNPs of 119 unrelated Taiwanese (50 men, 69 women) were sequenced using a commercial MPS kit. Forty-eight nondegraded and 44 highly degraded two-person artificial DNA mixtures with various minor to major ratios (1:9, 1:19, 1:29, 1:39, 1:79, and 1:99) were analyzed to examine the performance of this system for detecting the alleles of the minor contributors in DNA mixtures. Likelihood ratios based on continuous model were calculated using the EuroForMix for DNA mixture interpretation. The STR and SNP genotypes of these 119 Taiwanese were obtained. Several sequence variants of STRs were observed. Using EuroForMix software based on the sequence data of autosomal STRs and autosomal SNPs, 97.9% (47/48) and 97.7% (42/43) of minor donors were accurately inferred among the successfully analyzed nondegraded and degraded DNA mixtures, respectively. In conclusion, combined with EuroForMix software, this commercial kit is effective for assignment of the minor contributors in nondegraded and degraded DNA mixtures. |
| Databáze: | OpenAIRE |
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