The interplay between antimicrobial activity and reactivity of isothiocyanates
| Autor: | Carla Araya-Cloutier, Jean-Paul Vincken, Heidy M.W. den Besten, Leonie C. Waardenburg, Silvia Andini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Preservative Food spoilage 01 natural sciences Levensmiddelenmicrobiologie chemistry.chemical_compound Minimum inhibitory concentration 0404 agricultural biotechnology 010608 biotechnology Levensmiddelenchemie Food science Food Process Engineering Gram VLAG Growth medium biology Food Chemistry Chemistry Glucosinolate 04 agricultural and veterinary sciences biology.organism_classification Antimicrobial 040401 food science Fungicide Antibacterial Food preservative Brassicaceae Food Microbiology Bacteria Food Science Electrophilic |
| Zdroj: | LWT, 134 LWT 134 (2020) |
| ISSN: | 0023-6438 |
| Popis: | This study aimed to determine antimicrobial activity (minimum inhibitory concentration, MIC; minimum bactericidal/fungicidal concentration, MBC/MFC) of novel ITCs against food spoilage and pathogenic Gram– bacteria, Gram+ bacteria, and fungi. The activity of the long-chain (C9) 9-(methylthio)nonyl ITC (9-MTITC), 9-(methylsulfinyl)nonyl ITC (9-MSITC), and 9-(methylsulfonyl)nonyl ITC (9-MSoITC) was determined for the first time. Due to the electrophilicity of ITCs, the activity of ITCs was evaluated in nucleophile-rich and nucleophile-poor growth media. ITCs reacted via conjugation with components in a nucleophile-rich growth medium at a rate of 39–141 μmol L−1 h−1, depending on their side chain configuration and temperature. The reaction rates were lowered by a factor of 2–21 when using nucleophile-poor growth media. Consequently, the activity of ITCs was generally improved, with MSITC and MSoITC being the most positively affected (activity increased by a factor of > 4). 9-MSITC and 9-MSoITC had good activity (MIC ≤ 25 μg/mL) against Gram+ bacteria and fungi. The short-chained (C3) analogues had good activity against Gram+ bacteria and Gram– bacteria. The highest bactericidal/fungicidal activity was obtained for 9-MSITC and 9-MSoITC (MBC/MFC 17.5–25 μg/mL). Overall, MSITC and MSoITC might be potential new natural food preservatives, but their reactivity with food matrix components should be considered. |
| Databáze: | OpenAIRE |
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