Cerebrospinal fluid proteomics reveal potential protein targets of JiaWeiSiNiSan in preventing chronic psychological stress damage

Autor:	Ling Li, Han-Zhang Wang, Wu-Long Luo, Ning-Xi Zeng, Can Yan, Lili Wu, Hui-Zhen Li
Rok vydání:	2021
Předmět:	Male Proteomics Quantitative proteomics Pharmaceutical Science Hippocampus Context (language use) RM1-950 Pharmacology Immunofluorescence Cerebrospinal fluid Drug Discovery medicine Animals Rats Wistar Behavior Animal medicine.diagnostic_test Depression business.industry Dentate gyrus Stress resilience General Medicine UBA1 Resilience Psychological hippocampus neurogenesis Rats Disease Models Animal Complementary and alternative medicine neuron loss Chronic Unpredictable Mild Stress model Molecular Medicine Therapeutics. Pharmacology business Stress Psychological Drugs Chinese Herbal Research Article
Zdroj:	Pharmaceutical Biology article-version (VoR) Version of Record Pharmaceutical Biology, Vol 59, Iss 1, Pp 1065-1076 (2021)
ISSN:	1744-5116 1388-0209
Popis:	Context Chinese herbal formula JiaWeiSiNiSan (JWSNS) has been widely used to prevent stress-induced neuropsychiatric ailments in clinics and proven to have therapeutic anti-stress effects on rats. However, the mechanism remains unclear. Objective Based on the proteomics of cerebrospinal fluid (CSF), this study explores the possible mechanism and target proteins of JiaWeiSiNiSan raising stress resilience and preventing stress damage. Materials and methods A 6-week Chronic Unpredictable Mild Stress (CUMS) model was applied on adult Wistar male rats to observe the effects of JWSNS on improving mental stress resilience. Tandem Mass Tag (TMT) proteomics and bioinformatics analysis were used to screen and analyze differentially expressed proteins (DEPs) in CSF. Parallel Reaction Monitoring (PRM) was used to validate target DEPs. Results Significantly decreased sucrose preference, locomotion activity level and accuracy of T-maze, as well as increased immobility time, were observed in CUMS rats compared to CON rats while JWSNS improved above depression-like behaviours. The quantitative proteomics and bioinformatics analysis showed that JWSNS decreased the expression of Rps4x, HSP90AA1, Rps12, Uba1, Rsp14, Tuba1b in CUMS rats CSF (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6c3392d1aa403e385254bdcb8e49b4e https://doi.org/10.1080/13880209.2021.1954666 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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