Visualization of novel virulence activities of theXanthomonastype III effectors AvrBs1, AvrBs3 and AvrBs4

Autor: Boris Szurek, Ulla Bonas, Robert Szczesny, Norman Gürlebeck, Simone Hahn, Simone Jahn, Doreen Gürlebeck, Gerd Hause
Přispěvatelé: Institute of Biology, Martin-Luther-Universität Halle Wittenberg (MLU), Biocenter of the Martin Luther University Halle Wittenberg, Institute of Theoretical Physics, Charles University [Prague] (CU), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2009
Předmět:
0106 biological sciences
Transcription
Genetic
Nicotiana benthamiana
Plant Science
Vacuole
MESH: Virulence
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics
Phylogenetics and taxonomy
01 natural sciences
Type three secretion system
MESH: Plant Diseases
MESH: Tobacco
MESH: Bacterial Proteins
0303 health sciences
Virulence
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Effector
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
food and beverages
Xanthomonas campestris pv. Vesicatoria
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular Networks [q-bio.MN]
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
Xanthomonas campestris
Cell biology
MESH: Plant Leaves
Protein Transport
Subcellular Fractions
MESH: Cell Nucleus
MESH: Protein Transport
Xanthomonas
MESH: Ions
Soil Science
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Xanthomonas
Biology
MESH: Vacuoles
Microbiology
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Bacterial Proteins
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Tobacco
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Molecular Biology
Plant Diseases
030304 developmental biology
Cell Nucleus
Ions
[SDV.GEN]Life Sciences [q-bio]/Genetics
MESH: Transcription
Genetic
fungi
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Original Articles
biology.organism_classification
Plant Leaves
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
MESH: Subcellular Fractions
Vacuoles
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Molecular Plant Pathology
Molecular Plant Pathology, Wiley, 2009, 10 (2), pp.175-88. ⟨10.1111/j.1364-3703.2008.00519.x⟩
ISSN: 1364-3703
1464-6722
DOI: 10.1111/j.1364-3703.2008.00519.x
Popis: International audience; Xanthomonas campestris pv. vesicatoria secretes at least 20 effector proteins through the type III secretion system directly into plant cells. In this study, we uncovered virulence activities of the effector proteins AvrBs1, AvrBs3 and AvrBs4 using Agrobacterium-mediated transient expression of the corresponding genes in Nicotiana benthamiana, followed by microscopic analyses. We showed that, in addition to the nuclear-localized AvrBs3, the effector AvrBs1, which localizes to the plant cell cytoplasm, also induces a morphological change in mesophyll cells. Comparative analyses revealed that avrBs3-expressing plant cells contain highly active nuclei. Furthermore, plant cells expressing avrBs3 or avrBs1 show a decrease in the starch content in chloroplasts and an increased number of vesicles, indicating an enlargement of the central vacuole and the cell wall. Both AvrBs1 and AvrBs3 cause an increased ion efflux when expressed in N. benthamiana. By contrast, expression of the avrBs3 homologue avrBs4 leads to large catalase crystals in peroxisomes, suggesting a possible virulence function of AvrBs4 in the suppression of the plant defence responses. Taken together, our data show that microscopic inspection can uncover subtle and novel virulence activities of type III effector proteins.
Databáze: OpenAIRE
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