Association of chromosome 7 aneuploidy measured by fluorescence in situ hybridization assay with muscular invasion in bladder cancer
| Autor: | Xiayao Diao, Tianxin Lin, Weibin Xie, Lifang Huang, Hao Huang, Jianqiu Kong, Jian Huang, Chengran Huang, Shaoxu Wu, Junjiong Zheng, Haide Qin, Xiong Chen, Hao Yu, Jinhua Cai |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Oncology Cancer Research Multivariate statistics medicine.medical_specialty chromosome aneuploidy Aneuploidy preoperative prediction Logistic regression muscular invasion nomogram Correlation 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans fluorescence in situ hybridization In Situ Hybridization Fluorescence Aged Retrospective Studies Bladder cancer medicine.diagnostic_test Receiver operating characteristic business.industry Original Articles Middle Aged Nomogram medicine.disease 030104 developmental biology Urinary Bladder Neoplasms 030220 oncology & carcinogenesis Original Article Female business Chromosomes Human Pair 7 Fluorescence in situ hybridization |
| Zdroj: | Cancer Communications |
| ISSN: | 2523-3548 |
| Popis: | Background The preoperative prediction of muscular invasion status is important for adequately treating bladder cancer (BC) but nevertheless, there are some existing dilemmas in the current preoperative diagnostic accuracy of BC with muscular invasion. Here, we investigated the potential association between the fluorescence in situ hybridization (FISH) assay and muscular invasion among patients with BC. A cytogenetic‐clinical nomogram for the individualized preoperative differentiation of muscle‐invasive BC (MIBC) from non‐muscle‐invasive BC (NMIBC) is also proposed. Methods All eligible BC patients were preoperatively tested using a FISH assay, which included 4 sites (chromosome‐specific centromeric probe [CSP] 3, 7, and 17, and gene locus‐specific probe [GLP]‐p16 locus). The correlation between the FISH assay and BC muscular invasion was evaluated using the Chi‐square tests. In the training set, univariate and multivariate logistic regression analyses were used to develop a cytogenetic‐clinical nomogram for preoperative muscular invasion prediction. Then, we assessed the performance of the nomogram in the training set with respect to its discriminatory accuracy and calibration for predicting muscular invasion, and clinical usefulness, which were then validated in the validation set. Moreover, model comparison was set to evaluate the discrimination and clinical usefulness between the nomogram and the individual variables incorporated in the nomogram. Results Muscular invasion was more prevalent in BC patients with positive CSP3, CSP7 and CSP17 status (OR [95% CI], 2.724 [1.555 to 4.774], P |
| Databáze: | OpenAIRE |
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