Virus Adaptation and Selection Following Challenge of Animals Vaccinated against Classical Swine Fever Virus

Autor:	Martin Beer, Richard J. Orton, Dirk Höper, Thomas Bruun Rasmussen, Anders Gorm Pedersen, Ulrik Fahnøe, Camille Melissa Johnston, Jens Bukh, Graham J. Belsham
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine viral populations Deep sequencing Swine viruses Palatine Tonsil lcsh:QR1-502 lcsh:Microbiology Swine Diseases Haplotype selection education.field_of_study Virulence Vaccination High-Throughput Nucleotide Sequencing Adaptation Physiological 3. Good health Infectious Diseases Blood Viral evolution haplotype selection CSFV RNA Viral 030106 microbiology Population Viremia Classical swine fever virus Biology Vaccines Attenuated classical swine fever virus Polymorphism Single Nucleotide Virus Article Classical Swine Fever 03 medical and health sciences deep sequencing SDG 3 - Good Health and Well-being Virology Viral Interference medicine Animals education virus evolution Whole Genome Sequencing Haplotype Viral Vaccines biology.organism_classification medicine.disease Virus evolution Viral populations 030104 developmental biology Classical swine fever
Zdroj:	Viruses Viruses, Vol 11, Iss 10, p 932 (2019) Fahnøe, U, Pedersen, A G, Johnston, C M, Orton, R J, Höper, D, Beer, M, Bukh, J, Belsham, G & Rasmussen, T B 2019, ' Virus Adaptation and Selection Following Challenge of Animals Vaccinated against Classical Swine Fever Virus ', Viruses, vol. 11, no. 10, 932 . https://doi.org/10.3390/v11100932 Volume 11 Issue 10 Fahnøe, U, Pedersen, A G, Johnston, C M, Orton, R J, Höper, D, Beer, M, Bukh, J, Belsham, G J & Rasmussen, T B 2019, ' Virus adaptation and selection following challenge of animals vaccinated against classical swine fever virus ', Viruses, vol. 11, no. 10, 932 . https://doi.org/10.3390/v11100932
ISSN:	1999-4915
DOI:	10.3390/v11100932
Popis:	Vaccines against classical swine fever have proven very effective in protecting pigs from this deadly disease. However, little is known about how vaccination impacts the selective pressures acting on the classical swine fever virus (CSFV). Here we use high-throughput sequencing of viral genomes to investigate evolutionary changes in virus populations following the challenge of naï ve and vaccinated pigs with the highly virulent CSFV strain &ldquo Koslov&rdquo The challenge inoculum contained an ensemble of closely related viral sequences, with three major haplotypes being present, termed A, B, and C. After the challenge, the viral haplotype A was preferentially located within the tonsils of naï ve animals but was highly prevalent in the sera of all vaccinated animals. We find that the viral population structure in naï ve pigs after infection is very similar to that in the original inoculum. In contrast, the viral population in vaccinated pigs, which only underwent transient low-level viremia, displayed several distinct changes including the emergence of 16 unique non-synonymous single nucleotide polymorphisms (SNPs) that were not detectable in the challenge inoculum. Further analysis showed a significant loss of heterogeneity and an increasing positive selection acting on the virus populations in the vaccinated pigs. We conclude that vaccination imposes a strong selective pressure on viruses that subsequently replicate within the vaccinated animal.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6bd3a7716551d25f9c855fb210125d3 http://europepmc.org/articles/PMC6833067 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.