A novel approach to tag and identify geranylgeranylated proteins
| Autor: | Tamara Nyberg, Lea Guo, Brian Agnew, Courtenay Hart, Loren G. Fong, Lai N. Chan, Brandon S.J. Davies, Fuyuhiko Tamanoi, Stephen G. Young |
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| Rok vydání: | 2009 |
| Předmět: |
Electrophoresis
Proteomics Azides Fluorescence-lifetime imaging microscopy Clinical Biochemistry Protein Prenylation Biochemistry Article Mass Spectrometry Analytical Chemistry Mouse embryonic fibroblast Mice Prenylation Cell Line Tumor Animals Humans Electrophoresis Gel Two-Dimensional Protein Precursors Rhodamines Chemistry Nuclear Proteins Hydrogen-Ion Concentration Lamin Type A Progerin body regions Cell culture ras Proteins Click chemistry Protein prenylation |
| Zdroj: | ELECTROPHORESIS. 30:3598-3606 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.200900259 |
| Popis: | A recently developed proteomic strategy, the "GG-azide"-labeling approach, is described for the detection and proteomic analysis of geranylgeranylated proteins. This approach involves metabolic incorporation of a synthetic azido-geranylgeranyl analog and chemoselective derivatization of azido-geranylgeranyl-modified proteins by the "click" chemistry, using a tetramethylrhodamine-alkyne. The resulting conjugated proteins can be separated by 1-D or 2-D and pH fractionation, and detected by fluorescence imaging. This method is compatible with downstream LC-MS/MS analysis. Proteomic analysis of conjugated proteins by this approach identified several known geranylgeranylated proteins as well as Rap2c, a novel member of the Ras family. Furthermore, prenylation of progerin in mouse embryonic fibroblast cells was examined using this approach, demonstrating that this strategy can be used to study prenylation of specific proteins. The "GG-azide"-labeling approach provides a new tool for the detection and proteomic analysis of geranylgeranylated proteins, and it can readily be extended to other post-translational modifications. |
| Databáze: | OpenAIRE |
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