Partial sequencing and tissue distribution of the canine isoforms of steroid 5 alpha-reductase type I and type II
| Autor: | Paul N. Span, Jack A. Schalken, Anthony G. H. Smals, A. van Bokhoven, C.G.J. Sweep |
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| Rok vydání: | 2000 |
| Předmět: |
Gene isoform
medicine.medical_specialty Messenger RNA De etiologie van benign prostaathyperplasie Urology Biological activity Reductase Biology Molecular biology Isozyme Homology (biology) The etiology of Benign Prostatic Hyperplasia Role of the 5 alpha-reductase isozyme type I in the human prostate and its relation with Benign Prostatic Hyperplasia (BHP) Endocrinology Oncology Internal medicine Complementary DNA medicine De rol van het 5 alpha-reductase isozym type I in de humane prostaat en zijn betekenis voor het ontstaan van benigne prostaathyperplasie (BPH) Partial cloning |
| Zdroj: | Prostate, 44, 233-239 Prostate, 44, pp. 233-239 |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND The dog is regarded to be a valid model to test the effects of 5alpha-reductase inhibitors on prostatic growth. However, limited information is available on the characteristics or even existence of 5alpha-reductase isozymes in this species. METHODS Here, we set out to clone the cDNA of the dog isoforms of 5alpha-reductase type I and type II by a degenerate cloning strategy and to assess the tissue distribution of both transcripts and the enzymatic activity of the isozymes. RESULTS We identified two clones with homology to the human 5alpha-reductase isoforms type I and type II to be expressed in dog prostate. At the amino-acid level, these partial clones were found to exhibit a homology with their human counterparts of 83% and 88%, respectively. The expression levels of 5alpha-reductase mRNA were screened by RT-PCR in a number of dog tissues. No correlation was found between tissue mRNA expression and enzymatic 5alpha-reductase activities. CONCLUSIONS The present study describes the partial cloning of the dog 5alpha-reductase isozymes and their tissue distribution. These results provide additional data for the use of the dog as an animal model to investigate the role of 5alpha-reductase isozymes in steroid metabolism. |
| Databáze: | OpenAIRE |
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