Altered phenotype of HLA-G expressing trophoblast and decidual natural killer cells in pathological pregnancies

Autor: Peter M. Emmer, Kees Boer, Harold M.J. Kerstens, Eric A.P. Steegers, Johan Bulten, Willianne L.D.M. Nelen, Irma Joosten
Přispěvatelé: Obstetrics and Gynaecology
Rok vydání: 2002
Předmět:
Abortion
Habitual
medicine.medical_specialty
(Patho)Physiological
endocrinological and methabolic aspects [Prevention of disorders in human reproduction]
Cellular differentiation
Biology
Stem cell marker
Immunophenotyping
Natural killer cell
Andrology
Experimental diagnostics and therapy of malignancies
HLA Antigens
Pregnancy
Reference Values
Internal medicine
HLA-G
Recurrent miscarriage
Decidua
medicine
Humans
(Patho-)fysiologische
endocriene en metabole aspecten. [Preventie van stoornissen in de menselijke voortplanting]
Tumor pathology
HLA-G Antigens
Histocompatibility Antigens Class I
Rehabilitation
Obstetrics and Gynecology
Trophoblast
Placentation
Tumor pathologie
medicine.disease
Pregnancy
Ectopic
Trophoblasts
Killer Cells
Natural
medicine.anatomical_structure
Endocrinology
Reproductive Medicine
Female
Haematology
Zdroj: Human reproduction (Oxford, England), 17(4), 1072-1080. Oxford University Press
Human Reproduction, 17, 4, pp. 1072-80
Human Reproduction, 17, 1072-80
ISSN: 1460-2350
0268-1161
Popis: Item does not contain fulltext BACKGROUND: The interaction between decidual natural killer (NK) cells and alloantigens expressed on fetal trophoblast cells are thought to be essential for successful implantation and placentation. Consequently, a disturbed interaction during the first trimester of pregnancy might well lead to a subsequent pregnancy failure. METHODS: We investigated the expression of HLA-G and NK cell markers in tissue sections from recurrent miscarriage (n = 9) and ectopic tubal pregnancies (n = 5), and two hysterectomy specimens of healthy pregnancy as well as decidual biopsies (n = 9) were used as controls. RESULTS: We show in normal pregnancy not only a decrease, but also a morphological change in CD56+ NK cells upon interaction with HLA-G-expressing trophoblasts. The cells appear to be transitioning from a blast-like (activation) state into a state of apoptosis. The number of CD16+ NK cells was low. In contrast, in recurrent miscarriage tissue a sustained NK cell marker expression of both CD56 and CD16 was paralleled by a decreased expression of HLA-G. No morphological changes from the blast-like stage were apparent. Finally, in ectopic pregnancies HLA-G expression in the absence of decidual NK cells was associated with a disturbed trophoblast differentiation. CONCLUSIONS: In pathological pregnancies we show an in-situ altered phenotype of trophoblast and NK cells.
Databáze: OpenAIRE
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