Spectrofluorimetric determination of urinary p-aminobenzoic and p-aminosalicylic acids in the BT-PABA/PAS test of pancreatic function
| Autor: | R.W. Lobley, Joan M. Braganza, C. Chaloner, P. Gagjee, P.W. Pemberton |
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| Rok vydání: | 1991 |
| Předmět: |
Chromatography
Aminosalicylic acid Chemistry Hydrolysis Biochemistry (medical) Clinical Biochemistry Fluorescence spectrometry General Medicine Urine Aminosalicylic Acid Biochemistry High-performance liquid chromatography Colorimetry (chemical method) Excretion Pancreatic Function Tests chemistry.chemical_compound Spectrometry Fluorescence Pancreatitis para-Aminobenzoates Humans Dimethyl Sulfoxide 4-Aminobenzoic Acid Sodium acetate Chromatography High Pressure Liquid |
| Zdroj: | Clinica Chimica Acta. 199:253-262 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(91)90119-w |
| Popis: | Spectrofluorimetry was investigated as an alternative to HPLC for determining p-aminobenzoic acid and p-aminosalicylic acid in the N-benzoyl-L-tyrosyl-p-aminobenzoic acid/p-aminosalicylic acid test of pancreatic exocrine function. Urine specimens were hydrolysed for 30 min in 4 M NaOH at 100 degrees C. The fluorescence of p-aminobenzoic acid was measured in dimethyl sulphoxide solution (lambda ex = 300 nm, lambda em = 340 nm) and that of p-aminosalicylic acid in sodium acetate buffer, pH 4.0 (lambda ex = 297 nm, lambda em = 394 nm). The linear range was 0.038-8 mM for p-aminobenzoic acid and 0.051-12 mM for p-aminosalicylic acid, within-batch precision was 2.2% and 5.5%, respectively, and the entire analysis could be completed within 40 min. Although not eliminated, drug interference was greatly reduced in comparison with colorimetry. In 23 consecutive pancreatic function tests there was an excellent correlation between the p-aminobenzoic acid/p-aminosalicylic acid excretion index obtained by fluorimetry and the results from HPLC analysis (y = 0.914x + 0.070, r = 0.987, p less than 0.001). The method is simple, cost-effective and may be particularly valuable in developing countries having a high incidence of chronic pancreatitis. |
| Databáze: | OpenAIRE |
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