Immunocytochemical localization of cholecystokinin and glutamic acid decarboxylase during normal development in the prepyriform cortex of rats
| Autor: | A.E. Hendrickson, Lesnick E. Westrum, J.-Y. Wu, R. E. Westenbroek |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Aging Immunocytochemistry Glutamate decarboxylase Central nervous system Biology Immunoenzyme Techniques chemistry.chemical_compound Developmental Neuroscience Internal medicine medicine Limbic System Methods Animals Neurotransmitter Cholecystokinin Brain Chemistry Glutamate Decarboxylase Olfactory Pathways Cortex (botany) Rats Endocrinology medicine.anatomical_structure chemistry Cerebral cortex Lateral olfactory tract Developmental Biology |
| Zdroj: | Brain research. 431(2) |
| ISSN: | 0006-8993 |
| Popis: | Immunocytochemical localization of specific neurotransmitters in the brain is becoming increasingly important in studies of maturation. We have used the trilaminar prepyriform cortex (PC) of rats to study the distribution, patterns and relative number of cells, fibers and terminals during postnatal development using antisera to cholecystokinin (CCK) and glutamic acid decarboxylase (GAD). Both antisera show distinct patterns of immunoreactivity at birth and subsequent periods of distinct changes in these patterns. CCK immunoreactivity is rare but present at birth mostly in layer II. There is a dramatic increase of CCK-labeled structures between postnatal (PN) days 6 and 9 and between PN 13 and 21. The adult pattern is observed by PN 21 with large numbers of labeled cells in layer II, numerous terminals in layers II and deep I and large immunoreactive fibers in the lateral olfactory tract. At birth GAD-immunoreactive terminals are present mainly in layer I, forming a distinct pattern of superficial and deep bands. Subsequent major changes occur in this pattern between PN 9 and 13 and again between PN 13 and 21. By PN 21 there appears to be a loss in deeper laminae of GAD positive terminals which are possibly replaced by the increasing numbers of CCK terminals in the same sublaminae. The adult pattern of GAD immunoreactivity is established by PN 21 with terminals and a few cells in layer I. Therefore, throughout development of the rat PC, there is a distinct complementary and changing distribution of GAD and CCK. Factors that may influence these changes in immunoreactivity are discussed. |
| Databáze: | OpenAIRE |
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