Adhesion Molecules as Susceptibility Factors in Spontaneous Autoimmune Thyroiditis in the NOD-H2h4 Mouse
| Autor: | C. Lynne Burek, Noel R. Rose, Raphael Bonita, Linda Rasooly, Patrizio Caturegli |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Clinical Biochemistry Intercellular Adhesion Molecule-1 Thyroid Gland Vascular Cell Adhesion Molecule-1 Sodium Iodide Peripheral blood mononuclear cell Pathology and Forensic Medicine Autoimmune thyroiditis Mice Mice Inbred NOD Internal medicine medicine Animals Humans Molecular Biology ICAM-1 Chemistry Cell adhesion molecule Thyroid Thyroiditis Autoimmune medicine.disease Immunohistochemistry Lymphocyte Function-Associated Antigen-1 Disease Models Animal Mononuclear cell infiltration medicine.anatomical_structure Endocrinology Leukocytes Mononuclear Female Disease Susceptibility E-Selectin Lymphocytic Thyroiditis |
| Zdroj: | Experimental and Molecular Pathology. 73:155-163 |
| ISSN: | 0014-4800 |
| DOI: | 10.1006/exmp.2002.2470 |
| Popis: | Mononuclear cell infiltration of the thyroid is a prominent feature of chronic lymphocytic thyroiditis. Adhesion molecules play a major role in determining the localization of inflammatory mononuclear cells in the thyroid. Previous reports from animal models and human studies have described the thyroidal expression of adhesion molecules only late in clinical disease. In this study, we examined the distribution and kinetics of expression of E-selectin, VCAM-1, LFA-1, and ICAM-1 in the NOD-H2h4 mouse, a model of spontaneous autoimmune thyroiditis accelerated by dietary iodine. Mice were fed 0.015% NaI in their drinking water for 2, 4, 6, 8, and 16 weeks, and thyroids were removed, serially sectioned, and stained in an avidin–biotin–peroxidase assay. We found a dramatic increase in E-selectin and VCAM-1 expression on intrathyroidal endothelial cells after 16 weeks of iodine treatment. In addition, we describe for the first time that thyrocytes from the NOD-H2h4 mouse, and the parental NOD, constitutively express ICAM-1 independent of iodine treatment and prior to mononuclear cell infiltration of the thyroid gland. ICAM-1 was not detected on the thyrocytes of other untreated strains of mice, implicating expression of this adhesion molecule as a critical event in the recruitment of inflammatory mononuclear cells to the thyroid. |
| Databáze: | OpenAIRE |
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