Sedative-Hypnotic and Receptor Binding Studies of Fermented Marine Organisms
| Autor: | Sun Yong Chung, Hye Young Joung, Insop Shim, Bae Jin Lee, Young Mi Kang, Kyung Soo Kim |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
5-HT2C receptor
Pharmacology biology Lactobacillus brevis GABAA receptor Sedative-hypnotic activity biology.organism_classification Biochemistry Sleep in non-human animals Pentobarbital-induced sleep Oral administration Drug Discovery Sedative/hypnotic Insomnia medicine Molecular Medicine Original Article Fermented marine organisms medicine.symptom Receptor human activities |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 1976-9148 2005-4483 |
| DOI: | 10.4062/biomolther.2014.122 |
| Popis: | This study was performed to investigate the sedative-hypnotic activity of γ-aminobutyric acid (GABA)-enriched fermented marine organisms (FMO), including sea tangle (FST) and oyster (FO) by Lactobacillus brevis BJ20 (L. brevis BJ20). FST and FO were tested for their binding activity of the GABAA-benzodiazepine and 5-HT2C receptors, which are well-known molecular targets for sleep aids. We also measured the sleep latency and sleep duration during pentobarbital-induced sleep in mice after oral administration of FST and FO. In GABAA and 5-HT2C receptor binding assays, FST displayed an effective concentration-dependent binding affinity to GABAA receptor, similar to the binding affinity to 5-HT2C receptor. FO exhibited higher affinity to 5-HT2C receptor, compared with the GABAA receptor. The oral administration of FST and FO produced a dose-dependent decrease in sleep latency and increase in sleep duration in pentobarbital-induced hypnosis. The data demonstrate that FST and FO possess sedative-hypnotic activity possibly by modulating GABAA and 5-HT2C receptors. We propose that FST and FO might be effective agents for treatment of insomnia. |
| Databáze: | OpenAIRE |
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