Mangiferin and Hesperidin Transdermal Distribution and Permeability through the Skin from Solutions and Honeybush Extracts (

Autor:	Helena Baranska, Jadwiga Renata Ochocka, Justyna Stefanowicz-Hajduk, Krzysztof Cal, Anna Hering
Rok vydání:	2021
Předmět:	Adult Xanthones skin permeation Pharmaceutical Science skin penetration Administration Cutaneous High-performance liquid chromatography Article Analytical Chemistry chemistry.chemical_compound Hesperidin QD241-441 Drug Discovery Stratum corneum medicine Humans Physical and Theoretical Chemistry Mangiferin Transdermal Skin Ethanol Chromatography Cyclopia Plant Chemistry Plant Extracts Organic Chemistry Fabaceae Permeation Middle Aged Solutions medicine.anatomical_structure Microscopy Fluorescence Chemistry (miscellaneous) Polyphenol Molecular Medicine HPLC honeybush fluorescent microscopy
Zdroj:	Molecules Volume 26 Issue 21 Molecules, Vol 26, Iss 6547, p 6547 (2021)
ISSN:	1420-3049
Popis:	Polyphenolic compounds—mangiferin and hesperidin—are, among others, the most important secondary metabolites of African shrub Cyclopia sp. (honeybush). The aim of this study was to compare the percutaneous absorption of mangiferin and hesperidin from solutions (water, ethanol 50%, (v/v)) and extracts obtained from green and fermented honeybush (water, ethanol 50%, (v/v)). Research was performed with the Bronaugh cells, on human dorsal skin. The mangiferin and hesperidin distributions in skin layers (stratum corneum, epidermis, and dermis) and in acceptor fluid (in every 2, 4, 6, and 24 h) were evaluated by HPLC–Photodiode Array Coulometric and Coulometric Electrochemical Array Detection. The transdermal distribution of hesperidin was also demonstrated by fluorescence microscopy. Results indicated that mangiferin and hesperidin were able to cross the stratum corneum and penetrate into the epidermis and dermis. An advantage of hesperidin penetration into the skin from the water over ethanol solution was observed (451.02 ± 14.50 vs. 357.39 ± 4.51 ng/cm2), as well as in the mangiferin study (127.56 ± 9.49 vs. 97.23 ± 2.92 ng/cm2). Furthermore, mangiferin penetration was more evident from nonfermented honeybush ethanol extract (189.85 ± 4.11 ng/cm2) than from solutions. The permeation of mangiferin and hesperidin through the skin to the acceptor fluid was observed regardless of whether the solution or the honeybush extract was applied. The highest ability to permeate the skin was demonstrated for the water solution of hesperidin (250.92 ± 16.01 ng/cm2), while the hesperidin occurring in the extracts permeated in a very low capacity. Mangiferin from nonfermented honeybush ethanol extract had the highest ability to permeate to the acceptor fluid within 24 h (152.36 ± 8.57 ng/cm2).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6aaef6ca72b0d3fb00819e24fe27d55 https://pubmed.ncbi.nlm.nih.gov/34770957 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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