Creation and implantation of acellular rat renal ECM-based scaffolds
| Autor: | Andrea Peloso, Paolo Dionigi, Ilaria Benzoni, Massimo Abelli, Alessandro Malara, Carolina Bianco, Gaspari A, Jacopo Ferrario, Alessandra Balduini, Manuela Currao, Antonio Citro, Giuseppe Orlando, Marcello Maestri, Benedetta Maiga, Lorenzo Piemonti |
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| Přispěvatelé: | Pathology/molecular and cellular medicine, Peloso, Andrea, Ferrario, Jacopo, Maiga, Benedetta, Benzoni, Ilaria, Bianco, Carolina, Citro, Antonio, Currao, Manuela, Malara, Alessandro, Gaspari, Annalisa, Balduini, Alessandra, Abelli, Massimo, Piemonti, Lorenzo, Dionigi, Paolo, Orlando, Giuseppe, Maestri, Marcello |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology Embryology scaffold Kidney Regenerative medicine Extracellular matrix Kidney transplantation Tissue engineering Tissue Scaffold Decellularization Tissue Scaffolds Research Papers medicine.anatomical_structure tissue engineering Rat model Perfusion medicine.medical_specialty kidney bioscaffold extracellular matrix Biomedical Engineering kidney transplantation regenerative medicine Prosthesis Design Bioscaffold RATS Scaffold medicine Journal Article Animals Organ bioengineering Transplantation Tissue Engineering Cell-Free System business.industry Animal rat model medicine.disease Surgery Equipment Failure Analysi Equipment Failure Analysis Rats Inbred Lew organ bioengineering Rat business Developmental Biology |
| Zdroj: | Organogenesis |
| DOI: | 10.1080/15476278.2015.1072661 |
| Popis: | Kidney transplantation is the only potentially curative treatment for patient facing end-stage renal disease, and it is now routinely used. Its use is mainly limited by the supply of transplantable donor organs, which far exceeds the demand. Regenerative medicine and tissue engineering offer promising means for overcoming this shortage. In the present study, we developed and validated a protocol for producing acellular rat renal scaffolds. Left kidneys were removed from 26 male Lewis rats (weights: 250–350 g) and decellularized by means of aortic anterograde perfusion with ionic and anionic detergents (Triton X-100 1% and SDS 1%, respectively). 19 scaffolds thus obtained (and contralateral native kidneys as controls) were deeply characterized in order to evaluate the decellularization quality, the preservation of extracellular matrix components and resultant micro-angioarchitecture structure. The other 7 were transplanted into 7 recipient rats that had undergone unilateral nephrectomy. Recipients were sacrificed on post-transplantation day 7 and the scaffolds subjected to histologic studies. The dual-detergent protocol showed, with only 5 h of perfusion per organ, to obtain thoroughly decellularized renal scaffolds consisting almost exclusively of extracellular matrix. Finally the macro- and the microarchitecture of the renal parenchyma were well preserved, and the grafts were implanted with ease. Seven days after transplant, the scaffolds were morphologically intact although all vascular structures were obstructed with thrombi. Production and implantation of acellular rat renal scaffolds is a suitable platform for further studies on regenerative medicine and tissue engineering. |
| Databáze: | OpenAIRE |
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