Gene Expression, Oxidative Stress, and Senescence of Primary Coronary Endothelial Cells Exposed to Postprandial Serum of Healthy Adult and Elderly Volunteers after Oven-Cooked Meat Meals
| Autor: | Andrea Basso, Mauro Provinciali, Deborah Pacetti, Giacconi Robertina, Eugenio Mocchegiani, Francesco Piacenza, Michele Balzano, Marco Malavolta, Costarelli Laura, Natale G. Frega, Riccardo Gagliardi |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Volunteers 0301 basic medicine Senescence Aging medicine.medical_specialty Meat Article Subject DNA damage Immunology medicine.disease_cause 03 medical and health sciences Internal medicine Gene expression lcsh:Pathology Humans Medicine Cooking Cells Cultured Cellular Senescence Triglycerides Aged Regulation of gene expression Meal business.industry Endothelial Cells Cell Biology Middle Aged Postprandial Period Coronary Vessels Endothelial stem cell Oxidative Stress 030104 developmental biology Postprandial Endocrinology Gene Expression Regulation Reactive Oxygen Species business Oxidative stress Research Article lcsh:RB1-214 |
| Zdroj: | Mediators of Inflammation, Vol 2017 (2017) Mediators of Inflammation |
| ISSN: | 1466-1861 0962-9351 |
| DOI: | 10.1155/2017/3868545 |
| Popis: | Epidemiological studies have linked high consumption of meat with major age-related diseases including cardiovascular diseases. Abnormal postprandial increases in plasma lipids after a meat meal have been hypothesized among the pathogenetic mechanisms. However, it is still unknown if the postprandial serum derived after a normal meat meal is able to affect endothelial function, and if the type of meat and the age of the donors are critical factors. Here, we show the effects of postprandial sera derived from healthy adults and elderly volunteers who consumed meat meals on human coronary artery endothelial cell (HCAEC) oxidative stress, gene expression, DNA damage, and cellular senescence. We observed that a single exposure to postprandial serum induces a slight increase in ROS that is associated with modulation of gene expression pathways related to oxidative stress response and metabolism. The postprandial-induced increase in ROS is not associated with a measurable DNA oxidative damage. However, repeated exposure to postprandial serum induces an acceleration of cellular senescence. Taking into account the deleterious role of cellular senescence in age-related vascular diseases, the results suggest a new mechanism by which excessive meat consumption and time spent in postprandial state may affect health status during aging. |
| Databáze: | OpenAIRE |
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