Protection of Wistar-Furth rats against postischaemic acute renal injury
| Autor: | Anne-Emilie Decleves, Virginie Voisin, Nathalie Caron, Laetitia Giordano, Isabelle Habsch, Nadine Bouby, Vanessa Colombaro, Denis Nonclercq, Virginie Hubert |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Physiology Thromboxane medicine.medical_treatment Renal function Rats Inbred WF Inflammation Kidney Kidney Function Tests Nitric Oxide Real-Time Polymerase Chain Reaction Dinoprostone Nitric oxide chemistry.chemical_compound Thromboxane A2 Physiology (medical) Internal medicine medicine Animals Kidney transplantation Pharmacology biology business.industry Reverse Transcriptase Polymerase Chain Reaction medicine.disease Nephrectomy Nitric oxide synthase Thromboxane B2 Disease Models Animal Oxidative Stress Endocrinology chemistry Anesthesia Reperfusion Injury Acute Disease biology.protein medicine.symptom Nitric Oxide Synthase business Kidney disease |
| Zdroj: | Clinical and experimental pharmacology & physiology. 41(11):911-920 |
| ISSN: | 0305-1870 |
| Popis: | The Wistar-Furth (WF) rat strain is usually used in models of full major histocompatibility complex-mismatched kidney transplantation. Because these rats have been demonstrated to be resistant to several models of chronic kidney disease, the aim of the present study was to investigate their potential resistance to renal ischaemia-reperfusion (I/R) injury compared with another strain, namely Wistar-Hanover (WH) rats. Anaesthetized male WH and WF rats were submitted to I/R by occlusion of the left renal artery and contralateral nephrectomy. Urine, blood and tissue samples were collected at different time points after I/R to evaluate renal function, inflammation and tubular injury, along with determination of nitric oxide synthase (NOS) expression and thromboxane A2 (TxA2 ) production. Post-ischaemic renal function was better preserved in WF than WH rats, as evidenced by reduced levels of creatininaemia, urinary neutrophil gelatinase-associated lipocalin excretion and proteinuria. In addition, WF rats had less intrarenal inflammation than WH rats after I/R injury. These observations were associated with maintenance of neuronal NOS expression, along with lower induction of inducible NOS expression in WF versus WH rats. Moreover, WF rats excreted a significantly lower amount of TxB2 . The results indicate that WF rats are more resistant to an I/R injury than WH rats in terms of renal function and inflammation. These observations are associated with differential regulation of intrarenal NOS expression, as well as a reduction in thromboxane production, which could contribute to a better outcome for the postischaemic kidney in WF rats. |
| Databáze: | OpenAIRE |
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