The function of Notch signalling in segment formation in the crustacean Daphnia magna (Branchiopoda)
| Autor: | Petra Ungerer, Angelika Stollewerk, Bo Joakim Eriksson |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Embryo
Nonmammalian Pair-rule gene Notch signaling pathway Models Biological Segmentation cascade Spatio-Temporal Analysis biology.animal snail Delta-Notch signalling Animals Segmentation Gene Molecular Biology Body Patterning Receptors Notch Sequence Homology Amino Acid biology Gene Expression Regulation Developmental Vertebrate Hes Anatomy Cell Biology biology.organism_classification Crustacean Drosophila melanogaster Daphnia Evolutionary biology odd-skipped Pair-rule genes Arthropod Function (biology) Signal Transduction Developmental Biology |
| Zdroj: | Developmental Biology. 383(2):321-330 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2013.09.021 |
| Popis: | Ten years ago we showed for the first time that Notch signalling is required in segmentation in spiders, indicating the existence of similar mechanisms in arthropod and vertebrate segmentation. However, conflicting results in various arthropod groups hampered our understanding of the ancestral function of Notch in arthropod segmentation. Here we fill a crucial data gap in arthropods and analyse segmentation in a crustacean embryo. We analyse the expression of homologues of the Drosophila and vertebrate segmentation genes and show that members of the Notch signalling pathway are expressed at the same time as the pair-rule genes. Furthermore, inactivation of Notch signalling results in irregular boundaries of the odd-skipped-like expression domains and affects the formation of segments. In severe cases embryos appear unsegmented. We suggest two scenarios for the function of Notch signalling in segmentation. The first scenario agrees with a segmentation clock involving Notch signalling, while the second scenario discusses an alternative mechanism of Notch function which is integrated into a hierarchical segmentation cascade. |
| Databáze: | OpenAIRE |
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