Inhibition of cellular proliferation after experimental balloon angioplasty by low-molecular-weight heparin
| Autor: | Hartmut Hanke, K. R. Karsch, K. M. Schmid, S. Hassenstein, Martin Oberhoff, E. Betz, S. Hanke, J. Kamenz |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class medicine.medical_treatment Urology Low molecular weight heparin Balloon Muscle Smooth Vascular Restenosis Recurrence In vivo Physiology (medical) Angioplasty medicine Animals Carotid Stenosis Chemotherapy business.industry Heparin Heparin Low-Molecular-Weight medicine.disease Surgery Rabbits Carotid Artery Injuries Cardiology and Cardiovascular Medicine business Complication Angioplasty Balloon Cell Division medicine.drug |
| Zdroj: | Circulation. 85:1548-1556 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/01.cir.85.4.1548 |
| Popis: | BACKGROUND The proliferative response induced by balloon angioplasty is known to be an important factor in the development of restenosis after successful coronary angioplasty. METHODS AND RESULTS To study the effects of low-molecular-weight heparin (LMWH) on cellular proliferation after experimental balloon angioplasty, LMWH (3.9 kd, 400 anti-Xa units/kg/day) was given to 20 male New Zealand White rabbits. After an intimal fibromuscular plaque was induced by electrical stimulation in the right carotid artery, LMWH was applied during the 7 days after balloon dilatation. As the control group, 20 other rabbits underwent balloon angioplasty without application of LMWH. The vessels were excised 3, 7, 14, and 28 days after balloon treatment. During the final 18 hours before the rabbits were killed, bromodeoxyuridine was applied. Intimal wall thickness increased from 13 +/- 5 cell layers (preangioplasty control group) to 20 +/- 6 cell layers in the LMWH-treated group at 28 days (p less than 0.05). In contrast, histological examination of control animals 28 days after angioplasty revealed a significant increase to 35 +/- 15 cell layers (p less than 0.01). Immunohistological quantification showed a significant increase (p less than 0.001) of cells undergoing DNA synthesis at 3 (10.2 +/- 4.2%) and 7 (7.7 +/- 4.8%) days after balloon dilatation in control animals. In contrast, at 3 and 7 days after balloon treatment, the percentage of cells undergoing DNA synthesis in LMWH-treated rabbits was lower (3 days, 2.7 +/- 1.8%; 7 days, 1.9 +/- 0.3%) than the corresponding untreated controls but showed a significant increase (p less than 0.01) compared with the preangioplasty controls. The differences between the two groups were statistically significant, however (3 days, p less than 0.01; 7 days, p less than 0.05). As early as 14 days after angioplasty, the extent of cellular proliferation was normalized and was comparable to the preintervention levels in both groups. CONCLUSIONS Our data indicate that the proliferative response after balloon angioplasty can be reduced in vivo by early treatment with LMWH and thus encourage further clinical investigations. |
| Databáze: | OpenAIRE |
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