Genetic Typing of Feline Rabies Virus Isolated in Greater Bangkok, Thailand
| Autor: | Sununta Puempumpanich, Visith Sitprija, Wachiraporn Saengseesom, Songsri Kasempimolporn, Thaweesak Tirawatnapong |
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| Rok vydání: | 2004 |
| Předmět: |
Rabies
viruses Immunology Biology Cat Diseases medicine.disease_cause Microbiology Dogs Polymorphism (computer science) Virology medicine Animals Dog Diseases Deoxyribonucleases Type II Site-Specific Nucleocapsid Gene Molecular Epidemiology CATS Molecular epidemiology Rabies virus Nucleocapsid Proteins Thailand Nucleoprotein Genetic typing Cats Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Microbiology and Immunology. 48:307-311 |
| ISSN: | 0385-5600 |
| Popis: | To study the molecular epidemiology of rabies virus that is prevalent among cats in greater Bangkok, Thailand, a total of 17 rabies virus isolates from cats were characterized and compared with 120 rabies virus isolates from dogs. Analyses were performed on the genetic polymorphism in the rabies virus nucleoprotein (N) gene. Rabies virus N gene of isolates was amplified by reverse transcription-polymerase chain reaction. The diversity of N gene was revealed by the restriction fragment length polymorphism (RFLP) method. The rabies virus isolates from cats could be classified into 5 types, designated as Dd I-Hf I, Dd II-Hf II, Dd III-Hf I, Dd IV-Hf I, and Dd IV-Hf III. Type Dd I-Hf I was encountered more frequently than the others. It was apparent that no less than five rabies virus types presented in the areas of Bangkok. Moreover, all five RFLP patterns were typical of those which had been observed in dogs. Our findings suggest that there had been viral transmission between the dogs and the cats. |
| Databáze: | OpenAIRE |
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