Expression analysis of the exo-β-1,3-glucanase from the mycoparasitic fungus Trichoderma asperellum
| Autor: | Silvana P. da Silva, Roberto Nascimento Silva, César Marcos Marcello, Cirano José Ulhoa, Luiz Artur Mendes Bataus, Andrei Stecca Steindorff |
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| Rok vydání: | 2010 |
| Předmět: |
Trichoderma
Regulation of gene expression Fungal protein biology food and beverages Glucan 1 3-beta-Glucosidase Glucanase biology.organism_classification Microbiology Gene Expression Regulation Enzymologic Enzyme assay Fungal Proteins Rhizoctonia solani Gene Expression Regulation Fungal Gene expression Extracellular biology.protein |
| Zdroj: | Microbiological Research. 165(1):75-81 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/j.micres.2008.08.002 |
| Popis: | The regulation of the gene encoding the extracellular exo-beta-1,3-glucanase (tag83) produced by the mycoparasite Trichoderma asperellum was studied. Enzyme activity was detected in all carbon sources, but the highest levels were found when starch and purified cell walls from Rhizoctonia solani were used. These results are supported by the appearance of one strong band with enzyme activity in non-denaturing PAGE. Experiments using RT-PCR showed that exo-beta-1,3-glucanase induction in T. asperellum occurred at the transcriptional level. We used RT-PCR and real-time PCR analysis to examine the expression of tag83 gene during in vivo assay of T. asperellum against R. solani. We showed that the expression of tag83 is significantly increased by the presence of R. solani. |
| Databáze: | OpenAIRE |
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