Volatile self-inhibitor of spore germination in pathogenic Mucorale Rhizopus arrhizus
| Autor: | Rachna Singh, Mahaldeep Kaur |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Ecology biology 030106 microbiology Rhizopus oryzae Spores Fungal biology.organism_classification Applied Microbiology and Biotechnology Microbiology Culture Media Spore Rhizomucor pusillus 03 medical and health sciences 030104 developmental biology Rhizopus Germination Spore germination Rhizopus arrhizus Rhizomucor Mycelium |
| Zdroj: | FEMS Microbiology Ecology. 96 |
| ISSN: | 1574-6941 |
| Popis: | Rhizopus arrhizus is a common pathogenic Mucoralean mold that exists as a saprophyte, and is disseminated through sporangiospores, which germinate to form mycelia under suitable environmental or infection settings. Such morphological transitions are often mediated by self-produced effector molecules in a density-dependent fashion. This study aimed to elucidate if a quorum-dependent, cell-density-driven phenomenon exists in R. arrhizus, and identify the molecule(s) involved. The germination of R. arrhizus was observed to be reliant on the seeding density, with nearly 71% and 47% germination in Sabouraud dextrose and glucose asparagine media respectively at 1 × 105–1 × 106 spores/mL, and only 10% and 1% germination respectively with 1 × 108 spores/mL. The late-growth-stage supernatant also hindered the spore germination and liquid-culture biomass in a dose-dependent way. These effects were being mediated by a volatile inhibitor present in the headspace and supernatant of R. arrhizus cultures, identified as 2-methyl-2-butene by gas chromatography and electron ionization-quadrupole mass spectrometry. The compound was present in a density-dependent manner and considerably impaired fungal germ-tube emergence and elongation during germination. Spore swelling remained unaffected. Multiple thin protrusions comprising of F-actin and microtubules were seen emanating from the treated cells, suggestive of filopodia-like and cytoneme-like extensions. The same compound was also detected in Rhizomucor pusillus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|