Genomic analysis of three Clostridioides difficile isolates from urban water sources
| Autor: | Cathrin Spröer, Isabel Schober, Gayle K. McEwen, Alex D. Greenwood, Ulrich Nübel, Hans-Peter Grossart, Daniela Numberger, Jörg Overmann, Martinique Frentrup, Boyke Bunk, Thomas Riedel |
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| Rok vydání: | 2019 |
| Předmět: |
Bacteriological Techniques
0303 health sciences Veterinary medicine Genotype Clostridioides difficile 030306 microbiology Sediment Genomics Sequence Analysis DNA Biology Polymerase Chain Reaction Microbiology Berlin 03 medical and health sciences Infectious Diseases Wastewater ddc:570 Sewage treatment Water Microbiology Urban water Institut für Biochemie und Biologie Genome Bacterial Phylogeny Clostridioides 030304 developmental biology |
| Zdroj: | Anaerobe. 56:22-26 |
| ISSN: | 1075-9964 |
| Popis: | We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed. (C) 2019 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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