Unusual diversity of human rotavirus G and P genotypes in India
| Autor: | Maharaj K. Bhan, Harish Rawat, P. A. Woods, J. R. Gentsch, Madhumati Ramachandran, Savitri Thakur, Ramesh Kumar, Lilita Bahl, Aarti Vij, Nirmala Kesari, S. S. Bhambal, Bimal Kumar Das, Roger I. Glass |
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| Rok vydání: | 1996 |
| Předmět: |
Diarrhea
Rotavirus Microbiology (medical) Genotype Molecular Sequence Data India Reoviridae medicine.disease_cause Asymptomatic Rotavirus Infections medicine Humans Typing Genetic variability Child Base Sequence biology Molecular epidemiology Infant Newborn Genetic Variation biology.organism_classification Virology DNA Viral medicine.symptom Oligonucleotide Probes Research Article |
| Zdroj: | Journal of Clinical Microbiology. 34:436-439 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.34.2.436-439.1996 |
| Popis: | Between April and December 1993, we determined P and G genotypes of group A rotavirus strains obtained from children admitted to diarrhea treatment centers in five Indian cities. From a total of 63 rotavirus-positive specimens, we identified 10 different strains with five different G genotypes and four distinct P types by using reverse transcription-PCR. The common worldwide strains G1P8, G2P4, G3P8, and G4P8 were underrepresented among Indian children (33%), whereas strains of P type 6 (G1P6, G2P6, G3P6, G4P6, and G9P6), which primarily infect asymptomatic newborns but are rare in children with diarrhea were common in India (43%). Of these, G9P6, a strain not previously reported to be found in children with diarrhea, was the most prevalent (22%). Eleven percent of the strains were nontypeable, and another 11% of the specimens had mixed infections. Using digoxigenin-labeled, genotype-specific hybridization probes, we confirmed all G9 strains and mixed infections tested and identified three nontypeable strains (one G9 and two P8). The epidemiological significance of G9 rotavirus strains, if confirmed in other settings, may have important implications for vaccine development. |
| Databáze: | OpenAIRE |
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