Analysis of tilianin and acacetin in Agastache rugosa by high-performance liquid chromatography with ionic liquids-ultrasound based extraction
| Autor: | Jinfeng Wei, Pengran Cao, Wenyi Kang, Jinmei Wang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Chromatography
biology Acacetin Chemistry(all) 010405 organic chemistry Extraction (chemistry) Analytical chemistry Agastache 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences High-performance liquid chromatography Agastache rugosa 0104 chemical sciences chemistry.chemical_compound Acetic acid chemistry Ionic liquid Methanol 0210 nano-technology |
| Zdroj: | Chemistry Central Journal. 10(1) |
| ISSN: | 1752-153X |
| DOI: | 10.1186/s13065-016-0223-7 |
| Popis: | Ionic liquid 1-butyl-3-methylimidazolium bromide-methanol-based ultrasonic-assisted extraction (ILUAE) was used to extract tilianin and acacetin from the aerial parts of Agastache rugose (A. rugose), and simultaneously determined by reversed phase high performance liquid chromatographic (RP-HPLC) method with ultraviolet detection (RP-HPLC-UV). An InertSustain RP-C18 column was used with the mobile phase consisting of methanol and 0.2% acetic acid as gradient elution at the detection wavelength of 332 nm. The flow rate was 0.8 mL/min, and the column temperature was 30 °C. Under the optimized conditions, tilianin and acacetin displayed good linearity in the ranges of 0.0595–4.76 and 0.0585–4.68 μg/mL, respectively, with the average recoveries being 96.93 and 97.88%, respectively. The method of ILUAE was compared with the traditional methods, it exhibited higher efficiency, higher reproducibility and environmental friendly in analyzing the active compounds in traditional Chinese medicines (TCMs). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|