| Autor: |
Andrea Alegre-Martí, Alba Jiménez-Panizo, Adrián Martínez-Tébar, Coralie Poulard, M. Núria Peralta-Moreno, Montserrat Abella, Rosa Antón, Marcos Chiñas, Ulrich Eckhard, Josep M. Piulats, Ana M. Rojas, Juan Fernández-Recio, Jaime Rubio-Martínez, Muriel Le Romancer, Álvaro Aytes, Pablo Fuentes-Prior, Eva Estébanez-Perpiñá |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Science Advances. 9 |
| ISSN: |
2375-2548 |
| DOI: |
10.1126/sciadv.ade2175 |
| Popis: |
Mutations of the androgen receptor (AR) associated with prostate cancer and androgen insensitivity syndrome may profoundly influence its structure, protein interaction network, and binding to chromatin, resulting in altered transcription signatures and drug responses. Current structural information fails to explain the effect of pathological mutations on AR structure–function relationship. Here, we have thoroughly studied the effects of selected mutations that span the complete dimer interface of AR ligand–binding domain (AR-LBD) using x-ray crystallography in combination with in vitro, in silico, and cell-based assays. We show that these variants alter AR-dependent transcription and responses to anti-androgens by inducing a previously undescribed allosteric switch in the AR-LBD that increases exposure of a major methylation target, Arg 761 . We also corroborate the relevance of residues Arg 761 and Tyr 764 for AR dimerization and function. Together, our results reveal allosteric coupling of AR dimerization and posttranslational modifications as a disease mechanism with implications for precision medicine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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