DNA flow cytometric values in bladder carcinoma biopsies obtained from fresh and paraffin-embedded material
| Autor: | Jan Egil Melvik, Erik Thorud, S. Lunde, A. B. Jacobsen, Sophie D. Fosså, Erik O. Pettersen |
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| Rok vydání: | 1988 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty medicine.diagnostic_test Biopsy Histological Techniques DNA Neoplasm General Medicine Biology Flow Cytometry medicine.disease Paraffin embedded Pathology and Forensic Medicine Flow cytometry chemistry.chemical_compound Urinary Bladder Neoplasms chemistry Paraffin Carcinoma medicine Humans Immunology and Allergy DNA |
| Zdroj: | APMIS. 96:25-29 |
| ISSN: | 1600-0463 0903-4641 |
| DOI: | 10.1111/j.1699-0463.1988.tb05263.x |
| Popis: | DNA-histograms were obtained by flow cytometry (FCM) of nuclei prepared from fresh bladder carcinoma biopsies and from 100 micron sections of paraffin-embedded material from the same biopsies. Linear regression analysis of ploidy values from fresh material versus those from paraffin blocks showed excellent correlation (R2 = 0.957). Owing to the high background, the paraffin-embedded material was less suited for analysis of S-phase fraction. It is concluded that DNA FCM of nuclei obtained from paraffin-embedded bladder carcinoma biopsies yields reliable results concerning ploidy, but does not permit evaluation of the S-phase fraction. |
| Databáze: | OpenAIRE |
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