Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
| Autor: | Vlada B. Urlacher, Marco Girhard, Davide Decembrino |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
enzymes
010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Lignans laccase chemistry.chemical_compound Biotransformation phenolic coupling Very Important Paper medicine CueO Escherichia coli Furans Molecular Biology Laccase chemistry.chemical_classification Oxidase test Full Paper Molecular Structure 010405 organic chemistry Escherichia coli Proteins Organic Chemistry Periplasmic space Full Papers 0104 chemical sciences Enzyme chemistry Biocatalysis Molecular Medicine biotransformation Oxidoreductases Copper Coniferyl alcohol |
| Zdroj: | Chembiochem |
| ISSN: | 1439-7633 1439-4227 |
| Popis: | Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich substrates with concomitant reduction of molecular oxygen to water. The multi‐copper oxidase/laccase CueO of Escherichia coli is responsible for the oxidation of Cu+ to the less harmful Cu2+ in the periplasm. CueO has a relatively broad substrate spectrum as laccase, and its activity is enhanced by copper excess. The aim of this study was to trigger CueO activity in vivo for the use in biocatalysis. The addition of 5 mM CuSO4 was proven effective in triggering CueO activity at need with minor toxic effects on E. coli cells. Cu‐treated E. coli cells were able to convert several phenolic compounds to the corresponding dimers. Finally, the endogenous CueO activity was applied to a four‐step cascade, in which coniferyl alcohol was converted to the valuable plant lignan (−)‐matairesinol. Simple and efficient: The endogenous E. coli laccase CueO was activated by the addition of copper ions to cells, which were subsequently used for oxidative phenolic coupling. As a proof of concept, CueO‐catalyzed dimerization of coniferyl alcohol was integrated into a multi‐enzyme cascade in E. coli to produce the plant lignan (−)‐matairesinol. |
| Databáze: | OpenAIRE |
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