Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions
| Autor: | Walter Bonani, L. Zubal, D. Maniglio, D. Renciuk, Josef Jancar, Riccardo Ceccato, Lucy Vojtová, C. Migliaresi, Aleš Hampl |
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| Rok vydání: | 2018 |
| Předmět: |
Circular dichroism
Materials science Polymers and Plastics General Chemical Engineering Kinetics collagen viscoelastic properti 02 engineering and technology lcsh:Chemical technology 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Co2 removal collagen fibrillogenesis lcsh:TA401-492 Materials Chemistry lcsh:TP1-1185 structural analysis Physical and Theoretical Chemistry Dissolution Organic Chemistry viscoelastic properties protein self-assembly 021001 nanoscience & nanotechnology Microstructure biocompatible polymers Electrophoresis Chemical engineering chemistry Chemical agents Carbon dioxide Biocompatible polymers lcsh:Materials of engineering and construction. Mechanics of materials 0210 nano-technology |
| Zdroj: | eXPRESS Polymer Letters, Vol 12, Iss 2, Pp 159-170 (2018) EXPRESS POLYM LETT. 2018, vol. 12, issue 2, p. 159-170. |
| ISSN: | 1788-618X |
| DOI: | 10.3144/expresspolymlett.2018.14 |
| Popis: | Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications. |
| Databáze: | OpenAIRE |
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