Efficient Hydrolysis of Raw Microalgae Starch by an α-Amylase (AmyP) of Glycoside Hydrolase Subfamily GH13_37
| Autor: | Suo Xu, Yazhong Xiao, Chao He, Hui Peng, Yi Gao, Lu Zhai, Peng Xu |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glycoside Hydrolases Starch Molecular Sequence Data Raw material 01 natural sciences Enzyme catalysis Substrate Specificity 03 medical and health sciences chemistry.chemical_compound Hydrolysis Bacterial Proteins Chlorophyta 010608 biotechnology Enzymatic hydrolysis Microalgae Glycoside hydrolase Amylase Food science Amino Acid Sequence biology Chemistry Plant Extracts Temperature food and beverages General Chemistry Kinetics 030104 developmental biology Biofuel Multigene Family biology.protein Biocatalysis alpha-Amylases General Agricultural and Biological Sciences Sequence Alignment |
| Zdroj: | Journal of agricultural and food chemistry. 66(48) |
| ISSN: | 1520-5118 |
| Popis: | Microalgae starch is receiving increasing attention as a renewable feedstock for biofuel production. Raw microalgae starch from Tetraselmis subcordiformis was proven to be very efficiently hydrolyzed by an α-amylase (AmyP) of glycoside hydrolase subfamily GH13_37 below the temperature of gelatinization (40 °C). The hydrolysis degree reached 74.4 ± 2.2% for 4% raw microalgae starch and 53.2 ± 1.7% for 8% raw microalgae starch after only 2 h. The hydrolysis efficiency was significantly stimulated by calcium ions. The enzyme catalysis of AmyP and its mutants (Q306A and E347A) suggested that calcium ions contributed to the hydrolysis of cyclic structures in raw microalgae starch by a distinctive calcium-binding site Ca2 of AmyP. The study explored raw microalgae starch as a new resource for cold enzymatic hydrolysis and extended our knowledge on the function of calcium in amylolytic enzyme. |
| Databáze: | OpenAIRE |
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