Resolution of the intermediates of de novo purine biosynthesis by ion-pair reversed-phase high-performance liquid chromatography
| Autor: | David Patterson, Robert G. Hards |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Purine
Chromatography Resolution (mass spectrometry) Organic Chemistry Ion chromatography General Medicine Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Biosynthesis chemistry Purines Ultraviolet light Spectrophotometry Ultraviolet Absorption (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 455:217-224 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(01)82120-8 |
| Popis: | An ion-pair reversed-phase high-performance liquid chromatographic procedure capable of resolving twelve of the fourteen intermediates of de novo purine biosynthesis is presented. The method utilizes isocratic elution and detection by ultraviolet light absorption. Separation of all twelve intermediates can be achieved in 90 min. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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