Studies on biochemical and biomedical properties of Conus betulinus venom
| Autor: | Balasubramanian Thangavel, Giji Sadhasivam, Ramya Rajasekaran, Abirami Pachaiyappan, Arumugam Muthuvel |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Hemolytic assay Hyaluronidase lcsh:Medicine Venom Phospholipase Conus medicine MTT assay chemistry.chemical_classification biology Conus betulinus Fibrinogenolytic lcsh:R Proteolytic biology.organism_classification Molecular biology Infectious Diseases Enzyme chemistry Biochemistry Chemical fingerprinting medicine.drug |
| Zdroj: | Asian Pacific Journal of Tropical Disease, Vol 4, Iss Sup 1, Pp 102-110 (2014) |
| ISSN: | 2222-1808 |
| DOI: | 10.1016/s2222-1808(14)60423-0 |
| Popis: | Objective To study the biochemical and biomedical properties of predatory gastropod Conus betulinus venom. Methods Molecular weight of the crude venom extract was determined by using SDS-PAGE. Toxicity studies were carried out using haemolytic and brine shrimp lethality assays. Fibrin plate assay and substrate SDS-PAGE were used to determine the effect of sample on fibrin(ogen)olytic and proteolytic activity. The FTIR characterisation and chemical fingerprinting of amino acid were done with HPTLC. Enzymatic activities like phospholipase and hyaluronidase were measured spectrophotometrically and calculated in units. Anticancer screening was carried out by MTT assay. Results Studies on this deadly venom revealed six different molecular weight proteins of medical significance ranging between 20.0 kDa and 97.4 kDa. The protein content was estimated as 0.9 mg/mL. Haemolytic activity in erythrocytes was recorded and LC50 (Artemia) at 31.5 μg/mL. Further the venom showed considerable enzymatic properties like gelatinolytic, caesinolytic, fibrinolytic and fibrinogenolytic activities. The hyaluronidase and phospholipase activities were recorded at meagre range. The venom exhibited significant activity against HeLa cell lines. Moreover the evolution of venom is the crucial nature of Conus peptides in their challenging ecosystem. Periodical study on these peptides will unveil more peptides of biomedical use. Conclusions Although quite a lot of works have dealt with paralytic effects of Conus venom, it still remains as an unexplored cocktail with promising molecules for drug development. |
| Databáze: | OpenAIRE |
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