Knockout of SlMS10 Gene (Solyc02g079810) Encoding bHLH Transcription Factor using CRISPR/Cas9 System Confers Male Sterility Phenotype in Tomato
| Autor: | Sangsu Bae, Ki Hong Nam, Ill-Sup Nou, Myong Kwon Kim, Kwon Kyoo Kang, Hyo Ju Lee, Yu Jin Jung, Yong-Gu Cho, Dong Hyun Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sterility Plant Science Genetically modified crops Biology medicine.disease_cause male sterility 01 natural sciences Article 03 medical and health sciences SlMS10 gene Meiosis lcsh:Botany medicine tomatoes Gene CRISPR/Cas9 Ecology Evolution Behavior and Systematics Genetics Mutation Tapetum Ecology Wild type food and beverages Phenotype lcsh:QK1-989 030104 developmental biology bHLH transcription factor 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 1189, p 1189 (2020) Plants Volume 9 Issue 9 |
| ISSN: | 2223-7747 |
| Popis: | The utilization of male sterility into hybrid seed production reduces its cost and ensures high purity of tomato varieties because it does not produce pollen and has exserted stigmas. Here, we report on the generation of gene edited lines into male sterility phenotype by knockout of SlMS10 gene (Solyc02g079810) encoding the bHLH transcription factor that regulates meiosis and cell death of the tapetum during microsporogenesis in the tomato. Twenty-eight gene edited lines out of 60 transgenic plants were selected. Of these, eleven different mutation types at the target site of the SlMS10 gene were selected through deep sequencing analysis. These mutations were confirmed to be transmitted to subsequent generations. The null lines without the transferred DNA (T-DNA) were obtained by segregation in the T1 and T2 generations. In addition, we showed that the cr-ms10-1-4 mutant line exhibited dysfunctional meiosis and abnormal tapetum during flower development, resulting in no pollen production. RT-PCR analysis showed that the most genes associated with pollen and tapetum development in tomatoes had lower expression in the cr-ms10-1-4 mutant line compared to wild type. We demonstrate that modification of the SlMS10 gene via CRISPR/Cas9-mediated genome editing results in male sterility of tomato plants. Our results suggest an alternative approach to generating male sterility in crops. |
| Databáze: | OpenAIRE |
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