Challenge of BALB/c Mice with Respiratory Syncytial Virus Does Not Enhance the Th2 Pathway Induced after Immunization with a Recombinant G Fusion Protein, BBG2NA, in Aluminum Hydroxide
| Autor: | Patricia Tournier, Ultan F. Power, Thien Ngoc Nguyen, Christine Andreoni, Jean Francois Haeuw, Nathalie Corvaia, Hans Binz |
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| Rok vydání: | 1997 |
| Předmět: |
Cellular immunity
Paramyxoviridae T-Lymphocytes medicine.medical_treatment Epitopes T-Lymphocyte Aluminum Hydroxide Respiratory Syncytial Virus Infections BALB/c Mice Viral Proteins Th2 Cells Immune system Adjuvants Immunologic Bacterial Proteins Viral Envelope Proteins medicine Animals Humans Immunology and Allergy Cells Cultured Interleukin 4 Mice Inbred BALB C Vaccines Synthetic HN Protein biology Vaccination Viral Vaccines Immunoglobulin E biology.organism_classification Virology Infectious Diseases Immunization biology.protein Interleukin-5 Antibody Peptides Adjuvant |
| Zdroj: | The Journal of Infectious Diseases. 176:560-569 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | The polypeptide of aa 130-230 of the G protein (G2Na) of respiratory syncytial virus (RSV) was fused to BB, the albumin-binding region of streptococcal G protein, producing BBG2Na, which induced protective immune responses in rodent models. Evaluation of the immune response in mice immunized with BBG2Na in the adjuvant alhydrogel revealed high amounts of interleukin (IL)-5 and some IL-4 in splenocytes restimulated in vitro. This is compatible with a Th2 response. The activation of the Th2 pathway in such mice was further supported by the detection of IL-5 and G2Na-specific IgE in vivo. Of interest, in contrast to immunization with formalin-inactivated RSV, immunization of mice with BBG2Na followed by intranasal RSV challenge did not lead to increased production of IL-5- or G2Na-specific IgE. However, IgG1- and IgG2a-specific antibodies were boosted. These results demonstrate that the Th2 pathway is not enhanced by RSV challenge in BBG2Na-immunized mice. |
| Databáze: | OpenAIRE |
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