Lipid determination in bone marrow and mineralized bone tissue: From sample preparation to improved high-performance thin-layer and liquid chromatographic approaches
| Autor: | Alexandrine During |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Linolenic acid Linoleic acid Bone tissue Biochemistry Analytical Chemistry Linoleic Acid Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Calcification Physiologic Bone Marrow medicine Animals Femur Chromatography High Pressure Liquid Phospholipids chemistry.chemical_classification Chromatography Arachidonic Acid 030102 biochemistry & molecular biology Organic Chemistry Fatty Acids Fatty acid Analytic Sample Preparation Methods General Medicine Lipids Rats 030104 developmental biology medicine.anatomical_structure chemistry Docosahexaenoic acid lipids (amino acids peptides and proteins) Arachidonic acid Bone marrow Chromatography Thin Layer Long chain fatty acid |
| Zdroj: | Journal of chromatography. A. 1515 |
| ISSN: | 1873-3778 |
| Popis: | In view of their key roles in the bone physiology (e.g., in the biomineralization process) and their potential implication in bone pathologies, an approach to study lipids in situ is needed. The aim of the present paper is to propose an original procedure to characterize lipids in both bone marrow (BM) and mineralized tissue (MT) compartments, taking into consideration sample preparation, lipid extraction and analytical issues, when using small sample size (≤ 0.5g of rat femurs). The potential contamination of the MT by marrow lipids and the poor accessibility of certain lipids from the MT - two major issues in bone handling - were taking care, respectively by performing two cleaning steps after BM removal and by adding a demineralization step to the overall lipid extraction protocol. For lipid analyses, a multi-one-dimensional HP-TLC method was developed to analyze the major neutral and polar lipids at once and showed an excellent resolution (for 15 standards) and a good precision (inter-day RSD |
| Databáze: | OpenAIRE |
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